Bile loss damages the cerebellar Purkinje cells in rats

Abstract

Aim: The aim of the study was the estimation of the structural and metabolic changes in cerebellar Purkinje cells of rats during the complete outside bile abduction. Material and methods: Experiments were performed on male Wistar rats weighing 200-250 g. In 26 rats the fistula of the common bile duct was made and all the bile secreted by the liver was collected through a plastic tube into the outside glass bile container. 20 animals of the control group underwent sham operation (no removal of bile). On the 1th, 3th, and 5th days after the operation the animals of the control and experimental groups were decapitated and cerebellum cortex samples were collected for histology and cytochemistry. Results: The loss of bile induced the gradual increase in structural and metabolic abnormalities of Purkinje cells resulting in severe, irreversible disturbances and even death of some of them. The gradual decrease in Purkinje cells size, the loss of their sphericity and elongation, followed by inhibition of succinate-, NADH-, glucose-6-phosphate-dehydrogenases and the activation of lactate dehydrogenase and the marker enzyme of lysosomes acid phosphatase were revealed. All the changes reflect the ratio of the processes of damage and adaptation in the affected neurons in bile absence in the body. Conclusion: Total loss of bile (on the 1st, 3rd, and 5th days) induces the gradual increase in structural and metabolic disturbances in cerebellum Purkinje cells and death of some of them.