Study of the antagonistic effect of probiotics in dysbiotic disorders in rat infectious colitis model

Abstract

Background. Clostridium diffcile (C. diffcile) is the most common etiological factor for antibiotic-associated diarrhea. The development of this type of infection can lead to serious complications such as pseudomembranous colitis. This condition is diffcult to treat and can lead to the persistent disability of working-age patients. Probio re and Probio re plus are modern high-dose probiotics. Objective – to determine the effect of the use of Probio re and Probio re plus for the treatment of dysbiotic disorders that occurred in the modelling of infectious colitis in rats. Material and methods. Clinically healthy white outbred rats weighing 200.0-250.0 grams were taken for the experiment. To create experimental dysbiosis, animals were immunosuppressed by intramuscular injection of cyclophosphamide: the daily dose was 0.6 mg/kg for 7 days. Thereafter, exogenous microbial loading was performed by introducing into the stomach 1 ml of a suspension of St. aureus, C. albicans and Cl. perfringens for 3 days. The animals were treated for 5 days starting from the 11th day of the experiment. Rats were divided into the groups of 6 animals: group 1 - Probio re; group 2 - Probio re plus; group 3 - placebo (water); group 4 - intact animals. Results. The antagonistic effects of Probio re and, to a greater extent, Probio re plus against pathogenic microorganisms were detected. These probiotics restored the indices of normal intestinal microflora and suppressed pathogenic and conditionally pathogenic microflora. Conclusios. Probio re and Probio re plus can be used for the treatment of the dysbiotic disorders associated with infectious colitis.